The study of normal and leukemia hematopoietic populations as a basis for differentiation leukemic and regenerating cells

Project leader: Oľga Babušíková
Project duration: 2007 - 2009

The study of normal and leukemic hematopoietic populations as a basis of differentiation of leukemic and regenerating cells The main task of this annotation represents the extension of our previous studies which resulted in multiparameter flow cytometry for precise description of membrane/intracytoplasmic markers of both, B-lymphoid and myeloid acute leukemia. The reproducible results for exact leukemia diagnosis were obtained from leukemia-associated aberrant immunophenotypes localized on the so-called ´empty spaces´, in which normal cells are not present and from cells location on CD45/SSC dot plots as well. The results are appropriate for more exact detection of minimal residual disease. Our preliminary results showed the normal B-cell hematopoiesis to be present in bone marrow of patients with leukemia after termination of individual therapeutic phases and transplantation. For morphological and immunophenotypic similarities of leukemia blasts and regenerating benign precursors their discrimination is necessary. Therefore, we shall primarily direct to detailed definition of physiological maturation phases of B-lymphocytes (hematogones) and myeloid precursors. We propose the project if performed successfully the proper discrimination of both, the leukemia blasts and regenerating benign precursors is expected and the more exact detection of leukemia cells in small frequencies is possible.

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