Effect of natural compounds isothiocyanates (ITCs) on cellular mechanisms and markers associated with aggressive phenotype and treatment responsiveness in breast and ovarian cancer.

Project leader: Ľubica Hunáková
Project duration: 2008 - 2010

Project proposal is aimed at basic research of ITC utilization in antitumor therapy. It includes characterization of their biological properties exploitable in chemotherapy or for modulation of chemotherapy process aimed at explanation of their mechanisms of action. Primarily, we will study cellular mechanisms responsible for synergy of ITC and cisplatin- and anthracycline- based chemotherapeutic compounds. Further we will characterize modulation of markers associated with aggressive cancer phenotype (MMPs, CA IX, PBR). Parallelly we will determine inhibitive effect of ITCs on cell proliferation and viability, HDAC activity, cell cycle modulation and proportion of apoptosis and autophagy on cell death. Finally we will correlate expression of CAIX, PBR as well as results of MDR1 genotyping and phenotypization on archived carcinoma samples with accessible clinical data, in particular treatment outcome. This can bring new relevant decision maker indicators for therapeutical approaches.

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