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Reversion of chemoresistance of human cancer stem cells

Project leader: Zuzana Kozovsk√°
Project duration: 2013 - 2016

The population of tumour cells is heterogenic and consists of several subpopulations. Cancer stem cells constitute one subpopulation which is more chemoresistant than other subpopulations and is responsible in a great rate for chemotherapy failure. In our project, we will study subpopulation of cancer stem cells originated in human colorectal adenocarcinoma as well as resistant cell lines derived from this type of carcinoma. Using method of immunomagnetic separation we will separate several subpopulations with the cancer stem cells markers. We will analyze expression profile of these subpopulations and we will try to decrease expression of identified genes with siRNA silencing method. We will analyze to what extent silencing of chemoresistance genes changes the status of tumour stem cells and how the silencing of key genes in cancer stem cells alters their chemoresistance and tumourigenicity.

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