Cytotoxic Effect of Engineered Mesenchymal Stromal Cells on Human Chemoresistant Tumour Cells and Cancer Stem Cells
Project leader: Miroslava Matúšková
Project duration: 2013 - 2016
Chemoresistance of tumour cells is a serious problem in cancer treatment. Resistance against conventionally used chemotherapeutics is typical for tumour-initiating cells (TIC) or cancer stem cells (CSC), which are responsible for relapse of the tumour and metastatic spread. The efficacy of targeted chemotherapy by genetically engineered mesenhymal stromal cells (MSC) was confirmed in many studies. The study evaluating the efficacy of the treatment on chemoresistant tumour cells or tumour-initiating cells by MSC expressing prodrug-converting genes was not published yet. Our goal is to evaluate the extent of cytotoxic bystander effect mediated by MSC expressing fusion yeast cytosine deaminase or Herpes simplex virus thymidine kinase on chemoresistant cells and cells expressing markers of tumour initiating cells.