Implementation of methods for analysis of epigenetic changes in genes involved in epithelial-mesenchymal transition in breast cancer
Project leader: Božena Smolková
Project duration: 2014 - 2017
Circulating tumour cells (CTCs) are associated with prognosis in variety of human cancers, including breast cancer (BC) and has been widely studied for the early detection of metastatic tumour spread. Tumour cells before intravasation can undergo partial or total epithelial mesenchymal transition (EMT). Recent investigations of the mechanisms controlling EMT revealed a significant epigenetic regulatory impact reflecting the reversible nature of EMT. The aim of this study is to implement methods for analysis of DNA methylation changes related to the process of EMT. We will determine DNA methylation of genes coding expression of epithelial and mesenchymal proteins as well as methylation profiles of 22 genes associated with the EMT processes. Detection of epigenetic changes in EMT associated genes may lead to the identification of new diagnostic and prognostic markers as well as new therapeutic targets for individualized therapy of breast cancer patients.