DNA methylation profiles in genes associated with breast cancer metasasising
Project leader: Ivana Fridrichová
Project duration: 2015 - 2018
More than 25% of breast cancer patients develop metastatic disease that represent the main cause of high mortality. The epigenetic changes in tumorigenesis have been intensively investigated and new knowledge in metastatic process could contribute to the improving of advanced disease management and identifying of the new therapeutic targets. In present project, we will evaluate the DNA methylation levels in genes associated with cell-cell adhesion (ADAM23, CXCL12), degradation of basement membrane and extracellular matrix (uPA) and regulation of the epithelial-mesenchymal transition (TWIST, SNAI1 a SNAI2) in 50 invasive breast cancer patients with metastatic lymph nodes. Quantitative analyses of DNA methylation in peripheral blood samples, tumour tissues, positive lymph nodes and circulating tumour cells allow to evaluate the role of specific methylation profiles in partial processes of invasivity and metastasising and clinical utility for metastatic potential monitoring.