Study of protective potential of synthesized phenylethanoid glycosides in the systems of mammalian cells and plasmid DNA
Project leader: Eva Horváthová
Project duration: 2016 - 2019
DNA damage associated with different changes at the genetic level of the cell is generally considered as the most important stimulus for the initiation of the multistage process of carcinogenesis. The study of protective effects of natural compounds and their analogs, which are frequently used in health protection and prevention, is therefore of great importance. In the proposed project we plan: 1. to prepare phenylethanoid glycosides and their analogues using chemical or less conventional enzymatic procedures; 2. to determine their antioxidant, chelating and reducing capacity using biochemical methods; 3. in experimental systems utilizing mammalian cells, primary rat hepatocytes and plasmid DNA to evaluate their protective potential against lesions induced by model mutagens and carcinogens; 4. to monitor the activity of important cellular enzymatic and nonenzymatic antioxidants. Compounds studied could be a part of preventive and therapeutic strategies aimed at fighting the civilization diseases.