The role of nuclear factor-1 in growth regulated expression of the human ANT2 gene.

Project leader: Peter Baráth
Project duration: 2006 - 2008

Adenine nucleotide translocator is located in the inner mitochondrial membrane. It catalysis the exchange of ATP for ADP and, therefore, supplying the cell with the energy from mitochondria. Human transocator is encoded by three different isogenes (ANT1-3) which are expressed in tissue specific manner and depending on the developmental and growth state of the cells. In our previous work, we studied ANT2 gene which is induced during transition of some cells from quiescent G0 stage into the cell cycle. The analysis of the ANT2 promoter region has revealed the presence of both activating and repressing elements. The latest results implicate transcription factor NF1 (nuclear factor-1) in repression of the ANT2 transcript in G0 stage. NF1 represents a family of transcription factors encoded by four isogenes (NF1-A, B, C, X) that exist in several splicing forms. They regulate the expression of large amount of genes in many tissues and play important role during cellular development and tumorigenic transformation of the cell. The aim of this project is to characterize the molecular mechanism of the NF1 repressive effect on the ANT2 promoter through the protein-protein interactions. Identification of the NF1 protein partners will not only describe the growth regulation of the ANT2 promoter but it will contribute to understanding of transcriptional regulation during cellular development.

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